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DFS CDMA Tool v2.04 DFS Cdma Tool Evolution 4 0 0 3 setup free downloadInteraction of ATP with a model myosin head based on the crystal structure of chicken smooth-breast myosin subfragment 1.
ATP is a non-hydrolyzable analogue of ATP and binds to myosin motor proteins in a manner similar to that of ATP. However, in contrast to ATP, it has no effect on the actin-activated ATPase activity of the myosin molecule. To clarify the mechanism by which ATP inhibits the motile function of myosin, we have studied the interaction of ATP with a model of the myosin head in the presence of the native actin filament by means of the velocity gradient gel electrophoresis technique. The transient kinetics of the binding of ATP to the myosin model was investigated by the reaction mixture containing either actin or myosin particles labeled with fluorescein isothiocyanate (FITC-actin or FITC-myosin). The results indicate that the dissociation rate of the ATP-myosin complex is faster than that of the ATP-actin complex. Furthermore, the dissociation rate of the ATP-myosin complex is faster than that of the ADP-myosin complex, while the ATP-actin complex exhibits almost no difference in the rate of dissociation with either ATP, ADP, or AMPPNP. These results suggest that the ATP molecule weakens the interaction between the myosin head and the actin filament to yield the free state of myosin, as proposed by the “sticky-hand” model.Q:
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Hi i am new in codeigniter. I created a simple project and then when i try to open the site it shows a 404 error not found but the path is showing correctly in the address bar.
I have created a project folder named mySite and in mySite i have a controller named test_controller and i have a method named index() in the test_controller.
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